TECHNOLOGY: CALYSTA Energy™ PLATFORM

Transforming Low Cost Feedstocks into
High Value Sustainable Products

Calysta Energy™ has applied its expertise in biological engineering along with core capabilities in DNA synthesis and directed evolution to enable development of metabolic pathways for the biotransformation of novel low-cost feedstocks into high value sustainable products. Calysta is particularly focused on developing enzymes and organisms capable of efficiently converting currently underused feedstocks to high value chemicals now produced from petroleum.

GPS Bioengineering Platform

Development of advanced industrial biotechnology processes and products requires methods able to analyze highly complex systems with multiple variables. Calysta’s technology platform applies design optimization methods routinely used in other engineering disciplines to biological engineering. The GPS bioengineering platform uses customized algorithms and other advanced tools to quickly convert candidate DNA sequences to testable genes.

PhyloGPS™: Finding Starting Activity

The Phylo GPS™ process enables rapid identification of a starting enzyme to serve as a basis for subsequent protein engineering activities. Researchers using this process to rapidly mine large genome databases for high potential subsets of candidate genes, enabling efficient synthesis and testing.

GeneGPS™: Ensuring Enzyme Expression

The GeneGPS™ codon optimization methodology allows for efficient and predictable expression of heterologous genes in a target host. This technology is critical for functional testing of mined sequences and building synthetic pathways in industrial host organisms.

ProteinGPS™: Enzyme Activity Meeting Commercial Specifications

The Protein GPS™ protein engineering system quickly and efficiently designs proteins with specific, commercially-relevant characteristics. Using key amino acid substitutions, bioinformatics-based mining of available sequence space and advanced machine-learning algorithms, significant performance improvements in target protein sequences can be obtained from sample sizes considerably smaller than those required with conventional directed evolution methods.

PathwayGPS™: Building Metabolic Systems for Industrial Performance

The Pathway GPS™ system builds on other GPS systems to create functionally improved genetic pathways. Using Calysta’s low-cost, high capacity gene synthesis technology, multi-component multi-gene pathways demonstrating optimal industrial-scale performance can be produced with a minimum number of assays.

Calysta Energy Methanotrophs

Methylococcus capsulatus
grown at 42°C on methane/oxygen